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Diagnosis of histoplasmosis is done by obtaining the patient's history and by seeing the fungi in samples taken from infected tissue and examined microscopically. However, the definitive diagnosis of histoplasmosis is done by isolating (growing) the fungus from one or more tissue samples (blood or sputum samples or biopsy samples from skin, bone marrow, liver, or other organs). Growth of the fungus differentiates similar diseases from each other (for example, blastomycosis from histomycosis). In addition, there are immunology-based tests that can detect fungi antigens present in urine. Another available test can detect antibodies directed against the fungi; this test indicates the person has been exposed to the fungi but does not determine active infection.
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