The doctor or health care practitioner may perform blood tests to determine which hormone level is low and to rule out other causes. The following tests may be performed:

• ACTH and Cortrosyn stimulation test
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• TSH and thyroxine test
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• FSH and LH and either estradiol or testosterone (whichever is appropriate for the patient)
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• Prolactin test
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• GH stimulation test

An MRI or CT scan of the pituitary gland may be obtained to determine if a tumor is present.

In children, x-rays of the hands may be taken to determine if bones are growing normally.

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