Skin and Wound Cultures
A skin or wound culture is a test to find and identify germs (such as bacteria, a fungus, or a virus) that may be growing on the skin or in a wound. A sample of skin, tissue, or fluid is collected from the affected area and placed in a container with a substance (called growth medium or culture medium) that helps organisms grow. If nothing important grows, the culture is negative. If something that can cause infection grows, the culture is positive. The type of organisms may be identified with a microscope, chemical tests, or both.
Most bacteria can grow in oxygen. They are called aerobic bacteria and usually are found in wounds close to the skin surface (superficial). Bacteria that cannot grow in the presence of oxygen (anaerobic) usually are found in deeper wounds and abscesses. A wound culture can find out whether bacteria are aerobic or anaerobic.
A fungal culture is done to find out if an infection is caused by a fungus. A viral culture can be done to find out whether an infection is caused by a virus.
Some types of bacteria that normally live on or in the body can cause an infection if they go to parts of the body where they are not normally found. For example, E. coli</i> bacteria are normally found in the colon and anus. But if E. coli bacteria spread from the anus to the urethra, the bacteria may cause a urinary tract infection (UTI).
If a skin or wound culture is positive, other tests may be done to help choose the best medicine to treat the infection. This is called sensitivity testing.
Culture samples may also be collected from the ear or eye, from open or closed sores, or from nails and hair.
Why It Is Done
A skin or wound culture is done to:
How To Prepare
You do not need to do anything before you have this test. If you are taking or have recently taken antibiotics, tell your doctor.
Talk to your doctor about any concerns you have regarding the need for the test, its risks, how it will be done, or what the results will mean. To help you understand the importance of this test, fill out the medical test information form(What is a PDF document?).
How It Is Done
To collect a tissue or fluid sample from a wound, a sterile swab is inserted into the wound. The health professional collecting the sample may press around the wound and gently turn the swab to collect as much tissue or fluid as possible. The swab is then placed into either an aerobic or anaerobic culture tube or both, depending on the type of organism suspected.
A needle may be used to collect fluid from a wound that is covered (scabbed-over) or from an abscess. The fluid is then placed in the culture tube.
Once a sample is collected, it is placed in a container with a substance (called growth medium or culture medium) that helps bacteria, fungus, or viruses grow.
Any bacteria, fungi, or viruses that grow will be identified with a microscope, chemical tests, or both. If sensitivity testing is done to help make decisions about treatment, more time will be needed.
How It Feels
If you have a sample of fluid or tissue collected from a wound, you may feel some pain when the sample is collected. You may feel a short, sharp sting if you are given a shot of anesthetic to numb the area where the culture sample will be taken.
There is a very slight risk of spreading some infections if a biopsy is needed to collect the sample.
A skin or wound culture is a test to find and identify germs (such as bacteria, a fungus, or a virus) that may be growing on the skin or in a wound.
Some types of bacteria, fungi, and viruses grow quickly in culture, and some grow slowly. Test results may take from one day to several weeks, depending on the type of infection suspected.
If test results are positive, sensitivity testing may be done help make decisions about treatment.
What Affects the Test
Reasons you may not be able to have the test or why the results may not be helpful include:
What To Think About
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