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Definition of RT-PCR

RT-PCR: a highly sensitive technique for the detection and quantitation of mRNA (messenger RNA). The technique consists of two parts:

  1. The synthesis of cDNA (complementary DNA) from RNA by reverse transcription (RT) and
  2. The amplification of a specific cDNA by the polymerase chain reaction (PCR).

RT-PCR has been used to measure viral load with HIV and may also be used with other RNA viruses such as measles and mumps.

Source: MedTerms™ Medical Dictionary
http://www.medicinenet.com/script/main/art.asp?articlekey=22766
Last Editorial Review: 6/14/2012

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