Definition of Intervening sequence

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Intervening sequence: Part of a gene that is initially transcribed from the DNA into the primary RNA transcript but then is excised (removed) from it when the so-called exxon sequences on either side of it are spliced together.

Intervening sequences, which are also called introns, are genetic sequences that intervene between the exxons. The DNA of a gene is thereby made up of an alternation of introns and exxons.