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Other infections can mimic sporotrichosis, so a doctor performs tests to confirm the diagnosis. These tests usually involve a biopsy of one of the nodules, followed by an examination of the biopsy material under a microscope to identify the pathogen. Other possible infections that are easy to confuse with sporotrichosis include the following:
The fungi can be distinguished from other organisms by culturing the fungus from lesions and then identifying its characteristic structure. This is usually done in special labs set up with trained technicians. There are other tests like fluorescent antibody identification of the fungi, but these are not widely available.